Friday, Apr 10, 2015

High quality DNA ladders and protein ladders

ExcelBand Enhanced 3-color Regular Range Protein Marker (PM2510) and FluoroBand 1 KB Plus (0.1-10 kb) Fluorescent DNA Ladder (DM3260)

ExcelBand Enhanced 3-color Regular Range Protein Marker (PM2510)
250 μl x 2


The PM2510 ExcelBand Enhanced 3-color Regular Range Protein Marker is a ready-to-use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 180 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). PM2510 ExcelBand Enhanced 3-color Regular Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Contents
Approximately 0.2~0.6 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).

Quality Control
Under suggested conditions, PM2510 ExcelBand Enhanced 3-color Regular Range Protein Marker resolves 10 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane.

Storage
4°C ≥ 3 months; -20°C ≥ 24 months

FluoroBand 1 KB Plus (0.1-10 kb) Fluorescent DNA Ladder (DM3260)
500 μl


The DM3260 FluoroBand 1 KB Plus (0.1-10 kb) Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct loading on gel. The DNA Ladder DM3260 is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easy referencing. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.

Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range
100 ~ 10,000

Concentration
86 μg/ 500 μl

Recommended loading volume
5 μl/ well

Storage
Protected from light
Room temperature ≥ 6 months
4°C ≥ 12 months
-20°C ≥ 36 months

For more information, please visit: http://www.smobio.com/..

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