Wednesday, Mar 25, 2015

Breakthrough cost savings for genotyping assays

KASP™ genotyping technology

KASP™ genotyping technology utilises a unique form of competitive allele-specific PCR (polymerase chain reaction) that enables highly accurate bi-allelic scoring of SNPs (single nucleotide polymorphisms) and InDels (Insertions and Deletions) at specific loci across a wide range of genomic DNA samples, including those of complex genomes. KASP technology delivers extremely high levels of assay robustness and accuracy with significant cost savings.
Superb accuracy and performance
  • Accuracy >99.8% based on independent assessment
  • Industry leading SNP & InDel assay conversion rate (>90%)

Tremendous flexibility
  • Flexible primer design which increases the rate of successful assay development
  • Supports low-, medium- and highthroughput studies and individual repeat assays
  • Compatibility with a wide range of liquid handling systems and thermal cyclers; signal can be read on most FRET (fluorescent resonant energy transfer) capable plate readers and qPCR machines

Breakthrough cost savings
  • KASP uses a universal reporting system where the labelled components are present in the KASP Master mix
  • Eliminates the need for expensive labelled assay-specific primers or probes
  • Requires only 10 ng DNA per sample per SNP (based on human genome size)
  • Low reaction volumes keep reagent costs to a minimum
  • Cost benefits enable you to perform more assays overall, improving the quality of your data

How it works

KASP genotyping workflow

Following completion of the KASP PCR, reaction plates are read and the data analysed using any cluster analysis viewing software. Detected signals are plotted as a graph, with samples of the same genotype clustering together. In our genotyping service laboratories, cluster analysis is performed using our proprietary Kraken software. An example of the data generated is shown below.

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