Wednesday, Mar 25, 2015

RNA isolation without the need of β -mercapthoethanol and DNase

NucleoSpin® RNA Plus

The NucleoSpin® RNA Plus kit integrates a new feature, the NucleoSpin® gDNA Removal Column. This Mini spin column removes quickly and efficiently genomic DNA without the need of DNase digestion.

In the first step cells and tissues are lysed without the need of β-mercapthoethanol or TCEP. The chaotropic salt included in the lysis buffer immediately inactivates RNases. The lysate is added to the NucleoSpin® gDNA Removal Column to clarify the lysate and to remove contaminating gDNA. After addition of the binding solution to the flow-through, the RNA is bound to the NucleoSpin® RNA Plus Column. Afterwards, two washing steps remove salts, metabolites, and macromolecular cellular components. High quality RNA is eluted with RNase-free H2O.
Application data

Efficient RNA recovery

RNA was isolated from HeLa cells (5 x 106) and mouse kidney (20 mg) with the NucleoSpin® RNA Plus (blue) and a competitor kit (red). The RNA recovery was quantified by qRT-PCR. Lower CT values indicates higher RNA yields.

Reliable genomic DNA removal

NucleoSpin® RNA Plus (blue) and a competitor kit (red) have been used for the isolation of RNA from HeLa cells (5 x 106) and mouse kidney (20 mg). Residual genomic DNA were measured by qPCR. The MN kit shows higher CT values indicating a more efficient genomic DNA removal. Higher CT values mean less genomic DNA.

RNA of high integrity

10 mg mouse kidney tissue was used for RNA isolation. Equal amount of RNA were analyzed on an Agilent Bioanalyzer. The RIN value is higher for the MN kit.

Sample materials and typical downstream applications
  • RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
  • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Order Information
Product Pres Specification REF
NucleoSpin® RNA Plus 10 NucleoSpin® RNA Plus Columns with Collection Tubes, NucleoSpin® gDNA Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers 740984.10
50 as above 740984.50
250 as above 40984.250

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