Sequel : High throughput, single molecule sequencing system
Tuesday, Aug 08, 2017
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Sequel : High throughput, single molecule sequencing system

Pacific biosciences

 
Pacific Biosciences, Sequel System is based on the proven Single Molecule, Real-Time (SMRT) technology and delivers about 7X more reads with 1 million zero-mode waveguides (ZMWs) per SMRT Cell. The Sequel System is ideal for projects such as rapidly and cost-effectively generating high-quality whole genome de novo assemblies.
 
Overview of SMRT technology

 

SMRT technology, provide sequencing with,

 

1. Long read lengths -Produce average reads of 10 kb, some reads >60 kb

.
 

2. Uniform coverage- No DNA amplification, Least GC content and sequence complexity bias

 

3. High Accuracy- Achieves >99.999% (QV50) -Lack of systematic sequencing errors, Simultaneous epigenetic detection

 

4. Characterize epigenome -No separate sample preparation required

 

With the 7x more read than Pacbio RSII system, Sequel now can deliver,
1. Average read length:  10-18 kb
2. Throughput per cell:  5-8Gb
3. SMRTcell per run: 1-16
4. Movie lengths: 30 minutes to 10 hours
Sequel system is new industrial standards for
-Comprehensive de novo assemblies
- Target all types of variants across relevant genomic regions
- Full-length isoform transcripts
- Resolution of complex populations
- Methylation profiles
For more information, please contact us or visit http://www.pacb.com 

 



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