Wednesday, Dec 04, 2013
Download Brochure

EpiGnome™ : preparation for your Methylation analysis

Epicentre® Biotechnologies

Epicentre's methyl-seq kit produces whole genome bisulfite sequencing libraries from only 50ng of genomic DNA. EpiGnome™ follows a unique "post-bisulfite conversion" library construction method which yields highly diverse libraries with uniform coverage. This "post-bisulfite conversion" library construction method uses bisulfite-treated single-stranded DNA as template for the subsequent addition of adapter sequences required for cluster generation and sequencing. Thus, single-stranded DNA fragments independent of size and position of strand breaks remain viable templates for library construction, eliminating the loss of fragments and the selection bias associated with a post-library- construction bisulfite conversion strategy

  • Pre-library bisulfite conversion
  • Highly diverse libraries
  • Uniform CpG, CHG, and CHH coverage
  • 1-day procedure
  • Only 50 ng into bisulfite-conversion


Application
  • Library Prep for Whole Genome Bisulfite Sequencing

Click here for Application notes



Bisulfite treated ssDNA is random primed using a polymerase able to read uracil nucleotides to synthesize DNA strands containing a specific sequence tag as shown in figure 1. The 3'-ends of the newly synthesized DNA strands are then selectively tagged with a second specific sequence tag using a patented procedure resulting in di-tagged DNA molecules with known sequence tags at its 5' and 3' ends. The di-tagged DNA is enriched in PCR resulting in dsDNA with the appropriate sequences required for sequencing on any Illumina platform.

For more information, please contact us or visit www.epibio.com

Top of Page

Home | About Us | What's New | Product | Promotion | Contact Us | eBiotrade.com

Copyright © 1999-2017 Gene Company Limited. All Rights Reserved.