Wednesday, November 1, 2006

LI-COR Odyssey Infrared Imaging

Protein Phosphorylation Analysis1


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The Odyssey System is a complete solution for in vitro, in vivo, and tissue section analysis - all on a single platform. Features such as simultaneous two-color detection make it possible for ratiometric analysis in vitro, enabling easier and more cost effective tests for probe specificity before in vivo imaging. Additional solutions such as the Odyssey MousePOD&trade streamline your molecular imaging workflow and make it easier to achieve your research goals.

The Odyssey delivers two-color protein analysis on one blot, for easy, accurate comparison. Simultaneous, two-color detection eliminates the variables in comparing gels, enabling faster, more precise measurement of protein phosphorylation.

In one recent study1, mast cells sensitized with anti-DNP IgE were reacted with DNP38-albumin for 5 minutes. Two-fold serial dilutions of total cellular lysate from resting (lanes 1 to 4) and activated (lanes 5 to 8) cells were separated by SDS-PAGE, transferred onto nitrocellulose membrane, and detected simultaneously with mouse antibodies recognizing only tyrosine-phosphorylated ERK (visible in red), and rabbit antibodies recognizing ERK regardless of its phosphorylation status (visible in green).

Reference
1. Huaxian Chen and D. Michael Olive, Licor Inc.

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